Now, let us study the street plate method of isolation. Here is a brief outline. First we will go over the materials and proprietary details. Next we will look at the method itself, the results and the purpose of the technique. The materials needed for the method are quite basic and inoculating loop a sterile petri dish with appropriate media which is usually auger plate, a Bunsen burner or an electrical incinerator and a marker. Before starting with the procedure, it's good to label the petri dish plate on the bottom with the lid closed.

The main things to label are the name of the microbe, the type of fogger used, the date and the initials of the person who's doing it. Also It is good to sterilize the inoculating loop just before obtaining a specimen of the bacteria. Once you have labeled the petri dish and sterilize the loop, also known as transfer loop, open the culture tube and pick up a small sample of the specimen. Start streaking the plane in the following manner in quadrants, starting in one quadrant and then sterilize the loop again when going to the next quadrant. Therefore, start in one quadrant and then from the previous quadrant. Previous streak we start another streak and from the edge of the second streak, start, Third Street and finally from the edge of the third Start the fourth streak.

This method can also be done in three streets instead of four. One of the results of the streak plate method will be the continuous dilution of the specimen until isolated colonies appear on the petri dish played with the streak in quadrant area having the largest amount of bacteria as seen here. In be lesser, in see further lesser and finally the diluted colonies isolated colonies in quadrant B. Apart from dilution the strict plate method allows for differentiation between bacterial species This is due to the unique characteristics of bacterial colonies for each species. calling her jurisdiction include shape, margin, color, size, among Others in this example some bacteria are circular and reddish, while others are circular and whitish. One cannot forget the possibility of contamination by fungus or other contaminants as seen over here in yellow, which may be the case with in this plate.

Samples can be taken from single colonies and then cultured onto a new plate or in liquid broth for further identification, or testing. In conclusion, the streak plate method is a rapid and simple method used for the isolation of microorganisms with this method a pure strain of bacteria is obtained from a single species. This method helps identify the bacteria due to the colony characterizations. The isolated bacteria can then be used in larger batches and use for further studies such as in biotechnology applications and for testing. Therefore, not only morphological but physiological characteristics of the bacteria can be studied and tested. This concludes the lecture on the street plate method.