Today's light microscopes are much more sophisticated compared to the microscopes of Zachary's Jensen and Robert Hooke. Let us begin with light microscopy. Here is a brief outline for light microscopy. In part one, we'll begin with the use of us definitions and parts of the light microscope. Then, we'll study the image formation and the light pathway. In part two concepts related to microscopy and other configurations of light microscopy will be covered.

Light microscope is one which allows light to pass directly to the lenses and specimen. Such a configuration is called bright field microscopy. Because this type of microscope has several lenses, it is also called a compound microscope. The light pass Have a brightfield microscope is very simple. The light pack consists of a light source which is a crime illumination light, commonly a halogen lamp in the microscope Stan. A condenser lens which focuses the light from the light source on to the sample or the specimen and the objective and ocular lenses which allowed to view the sample image.

Shown here are the basic parts of a microscope. The ocular and objective lenses shown here ocular and objective answers magnify the image of the specimen. The stage is the platform on which the specimen is mounted. The condenser, as the name suggests, condenses or concentrates the light coming from the illumination source. The focusing knobs allowed to focus the image in a coarse manner, the bigger one and the fine in a fine manner, the smaller one and the light which refers to the halogen bulb. This picture shows the light microscope in more detail.

I'll focus on the highlighted parts since the other parts were discussed already. The microscope has a base and a neck shown here. So when holding a microscope one should hold With one hand by the neck and the place the other hand under the bass. The focusing knobs are the course knob and the fine focus knobs. The course focus knob moves the stage up and down the Find us so more slightly. The coaxial stage controls help move the specimen in different directions on the stage to the right left forward and backward.

The iris diaphragm it regulates the amount of light admitted to the condenser lens and the nosepiece allows for change of the objective lenses. Visual light is projected through a condenser lens which focuses light into a sharp cone as seen over here. Light then passes through the opening in the stage. When hitting the glass slide the light is either reflected or refracted as it passes through the specimen. Next light passing through the specimen enters the objective lens to form a magnified intermediate image that is inverted from that of the specimen itself. That this intermediate image it becomes the object that is magnified by the ocular lens are also known as the IPS.

And that is what is finally seen by the observer. As you can notice the red line that start at the left of the specimen, our phone towards the right of the inverted magnified image. And orange lines that begin at the left side of the specimen are found on the right side of the inverted magnified image. This slide shows the same concepts in writing that had been just out described. The main thing is that the light enters the objective lens, forming an image that is magnified an intermediate inverted image and the ocular lens also known as the eyepiece magnifies the intermediate inverted image that was formed by the objective lens So in summary, light microscopy allows light to pass directly through the lenses and thus has a configuration of a bright field microscope. It has several lenses and therefore it's known as compound microscope.

The main parts of the light microscope are the ocular objective and condenser lenses, the stage so the ocular objective and condenser lenses, the stage the focus knobs, the iris diaphragm, the light source, the base, the neck, the nosepiece the observation to And stage controls shown over here. The objective lens forms a magnified inverted image and the ocular lens forms magnifies even further the inverted intermediate image that was formed by the objective lens. This concludes part one of the study of light microscopy.