Now that we have studied about microscopes and cell types, we will focus on staining techniques. We will begin by looking at simple staining and negative staining, then gram staining And finally, acid fast stain here is an outline for simple and negative staining. We will look at the dyes used for simple and negative staining, the simple staining procedure and examples and then the negative staining procedure and examples. The simple stain technique involves plotting a prepared specimen with a basic dye. Since the basic dye has a positive charge, whereas bacterial cells have a negative charge, the dye is attracted to the bacteria and thus stains the bacteria as seen in the schematic and the negative stain technique. acidic dye is used.

Since the acidic dye has a negative charge and the bacterial cells also have a negative charge. The dye is repelled and therefore the cell remains unstained. Leaving thier cells on a dark background. In the simple staining procedure, one needs to hit fix the sample onto the slide immerse the sample into a dye solution, usually methylene blue which is a basic dye. Then, a Maldon such as iodine is used that reacts with methylene blue to form insoluble colored precipitate. Once rinsed, the excess stain is washed away but the more than 10 the remaining stain remains and the specimen can then be observed under the microscope.

Seen here in An example of a simple stain of a self image is that of human cheek epithelial cells stained with methylene blue. The stain allows us to identify the nucleus of the cell, the cytoplasm and the delimitation of the cell membrane for each cell. As seen over here, shown here is an example of a simple stain of the bacteria bacillus Mega tyrian stain with methylene blue. Now, let us focus on negative staining procedure. For this procedure, one has to smear the sample onto a slide then apply niagra sin or India ink nitrosamine as a black synthetic dye that is acidic, India ink is black and Aqua suspension of carbon particles then one Either one of those stains are applied dry it and the result is lighter inclusions well contrasted with a dark background shown here is the negative staining of the capsule of a bacteria.

The bacteria stain in dark blue, as seen over here the capsule is in white because it remains unstained. Here the capsules and the background remains stained. Here is that same bacteria bacillus material that is negatively stained with Niagara sin dye. As you can see, the bacteria remains clear whereas the background remains dark. So, in summary Simple staining is accomplished by using a basic dye, which has a positive charge such as methylene blue, the positive charge of the dye is attracted to the negative charge of the bacterial surface surface. Negative staining is accomplished by using an acidic dye which is negatively charged such as Niagara sin and India ink.

The negative charge of the dye is repelled by the negative charge of the surface of the bacteria. This concludes our study of simple and negative staining. Shown here is an example of a simple stain of the bacteria bacillus Mega tyrian stain with methylene blue. Now, let us focus on negative staining procedure. For this procedure one has to smear the sample onto a slide. Then apply Niagara sin or India ink magazine as a black synthetic dye that is acidic.

India ink is black and Aqua suspension of carbon particles. Then once either one of those things are applied great and the result is lighter inclusions well contrasted with a dark background. Shown here is the narrative staining of the capsule of a bacteria. The bacteria stain in dark blue as seen over here. The capsule is in white because it remains unstable. Other capsules and the background remains stained.

Here is that same bacteria bacillus Mega tyria that is negatively stained with Niagara sin die. As you can see the bacteria remains clear, whereas the background remains dark. So, in summary, simple staining is accomplished by using a basic dye, which has a positive charge such as methylene blue, the positive charge of the dye is attracted to the negative charge of the bacterial surface surface. Negative staining is accomplished by using an acidic dye which is negatively charged such as Niagara sin and India ink. The negative charge of the dye is repelled by the negative charge of the surface of the bacteria This concludes our study of simple and negative staining